Glutathione, Tylenol & Your Health: What You Should Know Interleukin 4 deficiency protects mice from acetaminophen induced liver injury and inflammation by prevention of glutathione depletion Inflammation Research Springer Nature Link Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen metabolism Sex differences in glutathione metabolism and acetaminophen toxicity