Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Paracetamol (Acetaminophen) hepatotoxicity and its management. Full article: Metabolic phenotyping applied to pre clinical and clinical studies of acetaminophen metabolism and hepatotoxicity Sex differences in glutathione metabolism and acetaminophen toxicity The molecular mechanisms of acetaminophen induced hepatotoxicity and its potential therapeutic targets Guangwen Luo, Lili Huang, Zhaowei Zhang, 2023