Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health sagesteele @P_McCulloughMD While I dont know whether there is a connection between Tylenol and autism, I do believe that there is reason to STOP giving it to babies children. Glutathione is impacted by Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products